Kata Kunci

Abstrak

One of the critical stages in Hematoxylin-Eosin (H&E) staining is deparaffinization, which involves removing paraffin from tissue samples. Xylene is commonly used for this purpose, but it is toxic and hazardous if ingested or inhaled. An alternative to xylene is dishwashing soap, which contains surfactants. These surfactants lower the surface tension of water, facilitating the removal of paraffin from the tissue preparations. This study aims to compare the quality of H&E staining in rat liver tissues deparaffinized with xylene versus dishwashing soap at concentrations of 0.5%, 1.0%, 1.5%, and 1.7%. The research employs a true experimental design with a Posttest-Only Control Group approach. The results indicate significant differences in staining quality between xylene and the various dishwashing soap concentrations. The quality percentages for xylene and dishwashing soap concentrations were 100%, 64%, 68%, 88%, and 88%, respectively. Concentrations of 0.5% and 1.0% dishwashing soap showed significant differences compared to xylene, while concentrations of 1.5% and 1.7% did not show significant differences and had similar staining quality to xylene. Based on these findings, dishwashing soap at concentrations of 1.5% and 1.7% is recommended as a viable alternative to xylene for the deparaffinization stage in H&E staining.